Propagation of nephropathia epidemica virus in Mongolian gerbils.
نویسندگان
چکیده
Nephropathia epidemica virus, the etiological agent of hemorrhagic fever with renal syndrome in Scandinavia, was serially propagated in Mongolian gerbils (Meriones unguiculatus). Intracerebrally inoculated suckling gerbils developed subclinical infections, with viral antigen found in lung, brain, liver and spleen. The distribution of viral antigen was similar to that seen in experimentally infected bank voles (Clethrionomys glareolus), the principal wild rodent reservoir of nephropathia epidemica virus. This model provides a readily available animal host for the study of experimental nephropathia epidemica virus infection.
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Detection of specific serum immunoglobulin M in nephropathia epidemica (Scandinavian epidemic nephropathy) by a biotin-avidin-amplified immunofluorescence method.
A biotin-avidin-amplified indirect immunofluorescence method was used to demonstrate specific serum immunoglobulin M (IgM) antibodies in nephropathia epidemica, the Scandinavian type of hemorrhagic fever with renal syndrome. The antigen in the test was the cross-reacting agent of Korean hemorrhagic fever, Hantaan virus. Sixty-two serum samples from 15 patients with clinically typical nephropath...
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ورودعنوان ژورنال:
- Journal of virology
دوره 53 3 شماره
صفحات -
تاریخ انتشار 1985